Week 14..

Hellooooo.. this is already the 14th weeks think everyone should already get use to working life.. another 6 more weeks till the end everyone jia you! anyway this week is my turn to blog again have a nice day reading..

I have been doing the control assay since the last post and I had gotten the result. You might be wondering why I took so long to get just one result. Due to some mistakes make and repeating of the same experiment a few times in order to confirm the results. Anyway some of you asked me about showing the graph during my last post. Now i shall show you some of the graphs that i had plotted.

Graph A Graph B

Graph C Graph D

Different gain are shown in the graphs. The higher the gain value, the higher the reading due to the higher sensitivity of the machine(Tecan plate reader). From the raw data graph, the curves for positive control show increasing reading over time, while the negative controls are quite constant over time. After getting the raw data, i will plot them into ratio(which mean positive control/negative control). From there, i can decide which gain value and which concentration of cells will give me better stability and better reading.

From graph B and D, gain 40 will give a stability for 2 hours and gain 50 and 60 will give stability for 4 hours.

My next experiment will be on the standard inhibitor.. This is to find out how long it will take for the bacteria cells to be inhibited.

Brief steps:

1. Inoculate and take OD reading of S.aureus.

2. Serial dilute and dilute S.aureus culture.

3. Prepare 96-well plate, incubate overnight.

4. Prepare ampicillin of different concentration.

5 Add ampicillin into the 96-well plate.

6. Prepare Resazurin.

7. Add Resazurin into 96-well plate.

8. Take fluorescence reading at 1 hour interval.

Have a nice day reading!!

Justina

0605950E

TG01